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91.
Jung Hoon Choi Ki-Yeon Yoo Choong Hyun Lee Ok Kyu Park Bing Chun Yan Hua Li In Koo Hwang Jeong Ho Park Sung Koo Kim Moo-Ho Won 《Neurochemical research》2010,35(10):1599-1608
In the present study, we investigated age-related differences in neuronal progenitors in the gerbil main olfactory bulb (MOB)
using doublecortin (DCX), a marker for neuronal progenitors which differentiate into neurons in the brain. No difference in
the number of neuronal nuclei (NeuN)-immunoreactive neurons was found in the MOB at variously aged gerbils. At postnatal month
(PM) 1, DCX immunoreaction was detected in all layers of the MOB except for the olfactory nerve layer. At this time point,
DCX-immunoreactive cells (neuronal progenitors) were very abundant; however, they did not have fully developed-processes.
From PM 3, the number of DCX-immunoreactive neuronal progenitors was decreased with age. At PM 6, DCX-immunoreactive cells
showed very well-developed processes. In western blot analysis, DCX protein level in the MOB was highest at PM 1. Thereafter,
levels of DCX protein were decreased with age. In the subventricular zone of the lateral ventricle, the number of Ki-67-immunoractive
cells (proliferating cells) was also significantly decreased with age. In addition, increases of α-synuclein-immunoreactive
structures were observed in the MOB with age. These results suggest that decrease in DCX-immunoreactive neuronal progenitors
and its protein levels in the MOB with age may be associated with reduction of cell proliferation in the SVZ and with an increase
in α-synuclein in the MOB. 相似文献
92.
Hyuk Woo Lee Hea Ok Kim Won Jun Choi Sun Choi Jin Hee Lee Seul-gi Park Lena Yoo Kenneth A. Jacobson Lak Shin Jeong 《Bioorganic & medicinal chemistry》2010,18(19):7015-7021
We synthesized homologated truncated 4′-thioadenosine analogues 3 in which a methylene (CH2) group was inserted in place of the glycosidic bond of a potent and selective A3 adenosine receptor antagonist 2. The analogues were designed to induce maximum binding interaction in the binding site of the A3 adenosine receptor. However, all homologated nucleosides were devoid of binding affinity at all subtypes of adenosine receptors, indicating that free rotation through the single bond allowed the compound to adopt an indefinite number of conformations, disrupting the favorable binding interaction essential for receptor recognition. 相似文献
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Cytokine GM-CSF genetic adjuvant facilitates prophylactic DNA vaccine against pseudorabies virus through enhanced immune responses 总被引:4,自引:0,他引:4
Yoon HA Aleyas AG George JA Park SO Han YW Lee JH Cho JG Eo SK 《Microbiology and immunology》2006,50(2):83-92
Granulocyte/macrophage colony-stimulatory factor (GM-CSF) is an attractive adjuvant for a DNA vaccine on account of its ability to recruit antigen-presenting cells (APCs) to the site of antigen synthesis as well as its ability to stimulate the maturation of dendritic cells (DCs). This study evaluated the utility of GM-CSF cDNA as a DNA vaccine adjuvant for glycoprotein B (gB) of pseudorabies virus (PrV) in a murine model. The co-injection of GM-CSF DNA enhanced the levels of serum PrV-specific IgG with a 1.5-to 2-fold increase. Moreover, GM-CSF co-injection inhibited the production of IgG2a isotype. However, it enhanced production of an IgG1 isotype resulting in humoral responses biased to the Th2-type against PrV antigen. In contrast, the co-administration of GM-CSF DNA enhanced the T cell-mediated immunity biased to the Th1-type, as judged by the significantly higher level of cytokine IL-2 and IFN-gamma production but not IL-4. When challenged with a lethal dose of PrV, the GM-CSF co-injection enhanced the resistance against a PrV infection. This suggests that co-inoculation with a vector expressing GM-CSF enhanced the protective immunity against a PrV infection. This immunity was caused by the induction of increased humoral and cellular immunity in response to PrV antigen. 相似文献
96.
We have identified a novel Ca2+-signal sensing GTPase (643 amino acid residues with an estimated molecular mass of 79 kDa) from the Arabidopsis genome database. This protein contains a RHO-like GTPase domain at the N-terminus (15–184 amino acids) and two calcium-binding EF-hand motifs (199–227 and 319–347 amino acids, respectively). It has the capability to bind calcium and hydrolyze GTP; in addition, its GTPase activity is regulated by changes in Ca2+ concentration. The expression of this gene was induced by ABA and salt stresses, and specific knock-out mutants were highly sensitive to ABA and salt treatments. These findings suggest that this protein is a novel ABA- and salt stress-related Ca2+ signal transducer. 相似文献
97.
Kyung Ok Shin Jeong Ryae Jeon Ji Seon Lee Jong Yeon Kim Chu Hee Lee Soon Dong Kim Yeon-Su Yu Doo Hyun Nam 《Biotechnology and Bioprocess Engineering》2006,11(3):240-244
To develop a health-aid preparation of Chinese yam (Dioscorea batatas Decne), lactic acid fermentation was attempted using a mixed starter comprising ofLactobacillus acidophilus, Streptococcus thermophilus, andBifidobacterium bifidus. The anaerobic fermentation of a 5% Chinese yam flour suspension gave a uniform suspension of pH 4.35, containing 7.76×106 CFU/mL lactic acid bacteria (LAB), and which was found to be acceptable to the panel from a sensory assessment. During the
administration of the lactic acid fermented (LAF) Chinese yam to Sprague Dawley rats for 6 weeks, a smaller body weight gain,
but greater excretion of feces were observed, implying the creation of a healthy gastrointestine on the administration of
LAF Chinese yam, which was also confirmed by the gastrointestinal motility of the feed in rats fed on LAF Chinese yam. The
constipation induced by loperamide was further suppressed in a rat group fed on a LAF Chinese yam diet, which was qualified
from healthy gastrointestinal flora established by LAB. A serochemical analysis revealed a slight improvement in the blood
glucose, neutral lipid and total cholesterol concentrations on administration of LAF Chinese yam, suggesting LAF Chinese yam
could be served as a healthy-aid preparation, even for hyperglycemia or hyperlipidemia patients. 相似文献
98.
Kilimcioğlu AA Ozkol M Bayindir P Girginkardeşler N Ostan I Ok UZ 《Parasitology international》2006,55(4):273-275
A total of 1205 primary school children were examined for cystic echinococcosis in five villages of Manisa, Turkey, to evaluate the efficacy of diagnostic methods of this infection in community-based screening surveys. Six hundred and thirty children from three villages, examined by a portable ultrasound scanner, chest microfilm and serological methods (ELISA, indirect hemagglutination) in our previous study, were designated as Study Group 1; and 575 children, from two adjacent villages, examined by ultrasonography alone in the present study, were designated as Study Group 2. In Study Group 1, hepatic cystic echinococcosis was detected in two cases (0.3%) by ultrasonography, while 43 (8.9%) and 49 (10.1%) cases were found to be positive for cystic echinococcosis by ELISA and indirect hemagglutination, respectively. Three of 575 children (0.5%) were diagnosed with cystic echinococcosis (two hepatic and one renal involvement) by ultrasonography alone in Study Group 2; and lung lesions were later detected in both cases with liver involvement by chest radiography.
Our results suggested that serological tests may be beneficial in suspected cases for confirmation and differential diagnosis, but have some drawbacks, such as discrepancy in results and high false seropositivity rates. Chest microfilm is not easy in field studies and exposure to X-ray is undesirable. As a reliable, simple, inexpensive and rapid technique, ultrasonography alone is recommended to be used in community-based screening surveys for cystic echinococcosis with confirmatory tests for suspected cases found during the screening program. 相似文献
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